Erratum to: “I-SceI meganuclease mediates highly efficient transgenesis in fish” [Mech. Dev. 118 (2002) 91–98]
نویسندگان
چکیده
Violette Thermes, Clemens Grabher, Filomena Ristoratore, Franck Bourrat, André Choulika*, Jochen Wittbrodt*, Jean-Stéphane Joly* INRA Junior Group, UPR2197, Institut de Neurobiologie A. Fessard, CNRS, Avenue de la Terrasse, 91 198 Gif-Sur-Yvette, France Developmental Biology Programme, EMBL-Heidelberg, Meyerhofstrasse 1, 69012 Heidelberg, Germany Cellectis SA, 25 Rue du Docteur Roux, 75 724 Paris, France
منابع مشابه
I-SceI meganuclease mediates highly efficient transgenesis in fish
The widespread use of fish as model systems is still limited by the mosaic distribution of cells transiently expressing transgenes leading to a low frequency of transgenic fish. Here we present a strategy that overcomes this problem. Transgenes of interest were flanked by two I-SceI meganuclease recognition sites, and co-injected together with the I-SceI meganuclease enzyme into medaka embryos ...
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The meganuclease I-SceI has been effectively used to facilitate transgenesis in fish eggs for nearly a decade. I-SceI-mediated transgenesis is simply via embryo cytoplasmic microinjection and only involves plasmid vectors containing I-SceI recognition sequences, therefore regarding the transgenesis process and application of resulted transgenic organisms, I-SceI-mediated transgenesis is of mini...
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In this study, we report a highly efficient transgenesis technique for Xenopus tropicalis based on a method described first for Medaka. This simple procedure entails co-injection of meganuclease I-SceI and a transgene construct flanked by two I-SceI sites into fertilized eggs. Approximately 30% of injected embryos express transgenes in a promoter-dependent manner. About 1/3 of such embryos show...
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متن کاملThese De Doctorat De L ’ Universite Pierre Et Marie Curie
Target-site double-strand breaks (DSBs) at natural endogenous loci were shown to increase the rate of gene replacement by homologous recombination in mouse embryonic stem cells. The effect of DSB at the dopachrome tautomerase (Dct) locus on the efficiency of gene targeting was studied using an embryonic stem cell line carrying the recognition site for the yeast I-SceI meganuclease embedded in t...
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ورودعنوان ژورنال:
- Mechanisms of Development
دوره 120 شماره
صفحات -
تاریخ انتشار 2003